Volume 4, Issue 2, March 2016, Page: 18-25
Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors
Gabriel Guillén-Ruiz, Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Blandina Bernal-Morales, Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Carlos M. Contreras, Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México; Peripheral Unit, Biomedical Research Institute, National Autonomous University of México, Xalapa, Veracruz, México
Jonathan Cueto-Escobedo, Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Juan Francisco Rodríguez-Landa, Neuropharmacology Section, Institute of Neuroethology, University of Veracruz, Xalapa, Veracruz, México
Received: Mar. 8, 2016;       Accepted: Mar. 18, 2016;       Published: Mar. 31, 2016
DOI: 10.11648/j.ajpn.20160402.11      View  4349      Downloads  149
A mixture of eight fatty acids (linoleic, oleic, palmitic, stearic, myristic, elaidic, lauric, and palmitoleic acids) at similar concentrations that have been identified in human amniotic fluid exerts anxiolytic-like effects similar to diazepam in adult Wistar rats through actions at -aminobutyric acid-A (GABAA) receptors, but unknown is whether any of these fatty acids exerts a predominant action over the others in infant rats. Of these fatty acids, some actions of oleic acid have already been identified, and it is one of the most abundant in amniotic fluid. Therefore, the aim of this study was to explore the effect of oleic acid on anxiety-like behavior and motoric activity in infant rats. To explore sedative actions, 28-day-old Wistar rats received 80-320 µg oleic acid or a sedative dose of diazepam (5 mg/kg). In a dose-response study, other groups of rats were injected with 10-80 µg oleic acid or 1 mg/kg diazepam. In an interaction study, rats that received oleic acid were pretreated with the GABAA receptor antagonists picrotoxin or flumazenil to explore the participation of this receptor in the effects of oleic acid on behavior in the elevated plus maze, rotarod test, and open field test. Oleic acid produced sedative effects but did not exert any anxiolytic-like actions. Hypoactivity and motor incoordination that were induced by oleic acid were blocked by flumazenil and picrotoxin. In conclusion, oleic acid reduced locomotor activity and motor incoordination through actions at the GABAA receptor.
Hypoactivity, Motor Incoordination, Fatty Acids, Oleic Acid, Anxiety
To cite this article
Gabriel Guillén-Ruiz, Blandina Bernal-Morales, Carlos M. Contreras, Jonathan Cueto-Escobedo, Juan Francisco Rodríguez-Landa, Oleic Acid Produces Motor Incoordination and Hypoactivity in Infant Wistar Rats Through GABAA Receptors, American Journal of Psychiatry and Neuroscience. Vol. 4, No. 2, 2016, pp. 18-25. doi: 10.11648/j.ajpn.20160402.11
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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