Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of <i>Centella asiatica</i>

Md. Shohel Hossain; Fahmida Abdullah Tuly; Sharmin Akter; Md Saiful Islam Arman; Md. Abdul Aziz; Md. Ekhtear Mahmud; Palash Das; Mohammad Hasem Babu; Md. Monirul Islam

doi:doi:10.11648/j.plant.20180601.11

Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica

Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, Md. Ekhtear Mahmud, Palash Das, Mohammad Hasem Babu, Md. Monirul Islam

Published in Plant (Volume 6, Issue 1)

Received: 5 March 2018 Accepted: 24 March 2018 Published: 13 April 2018

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Abstract

This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).

Published in	Plant (Volume 6, Issue 1)
DOI	10.11648/j.plant.20180601.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Antiemetic, Antimicrobial, Antioxidant, Cytotoxicity, Centella asiatica

References

[1]	M. E. Cartea, M. Francisco, P. Soengas, and P. Velasco, “Phenolic compounds in Brassica vegetables,”Molecules., 16(1), 251-280, 2011.
[2]	S. J. Duthie, A. Ma, M. A. Ross, and A. R. Collins, “Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes,” Cancer Res., 56, 1291–1295, 1996.
[3]	M. T. H Tanna, A. K Nath, M. N. Amin, M. Ibrahim, M. I. Chowdhury, M. E. H. Mukul, M. S. U. Rashed, A. Kabir, M. Ahmed, and M. S. Hossain, “Evaluation of antioxidants, membrane stabilizing, cytotoxic and anthelmintic activity with phytochemical screening of Chromolaena odorata: A medicinal shrub”, Int J Pharm, 6(1):53-61, 2016.
[4]	J. D. Mc Chesney, S. K. Venkataraman, and J. T. Henry, “Plant natural products: back to the future or into extinction?,” Phytochemistry, 68(14), 2015-22, 2007.
[5]	A.Z. Fake, R. D. Scalley, and A. G. Bailey, “Practical selection of antiemetics,” Am Fam physician, 69, 1169–1174, 2004.
[6]	A. I. Sajib, S. M. R. Dewan, A. Das, M. S. Sarwar, R. C. Sarkar, M. Ahmed, and M. S. Islam, “Invitro antimicrobial activity study and invivo antiemetic, antinociceptive activity evaluation of leaves extract of Erioglossum rubiginosum using experimental animal model,” Oriental Pharmacy and Experimental Medicine, 15, 135-140, 2015.
[7]	M. Balouiri, M. Sadiki, and S. K. Ibnsouda, “Methods for invitro evaluating antimicrobial activity: A review,” Journal of Pharmaceutical Analysis, 6(2),71-79,2016.
[8]	Nascimento, G. F. Gislene, J.Locatelli, P. C. Freitas, and G. L. Silva, “Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria,” Braz. J. Microbiol. 31(4), 247-256, 2000.
[9]	K.J. Davies, “Oxidative Stress: The Paradoxof Aerobic Life,” Biochemical Society Symposium, 61, 1-31,1995.
[10]	T. M. Mustafa, M. K. Sura, M. N. J. Abdul kadir, and I. A. Sarah, “Free radical and human health,” International journal of innovation sciences and research, 4(6), 218-223, 2015.
[11]	A. K. Tiwari, “Imbalance in antioxidant defense and human diseases: Multiple approach of natural antioxidant therapy,” Curr.Sci., 81(9), 1179-1187, 2001.
[12]	K. Bagchi, and S. Puri, “Freeradicals and antioxidants in health and disease,” East. Mediterranean Health Jr., 4,350-360, 1998.
[13]	P. Montuschi, J. P. Barnes, and J. L. Roberts, “Isoprostanes: markers and mediators of oxidative stress,” The FASEB Journal, 18(15), 1791-1800, 2004.
[14]	N. Jose and K. K. Janardhanan, “Antioxidant and antitumor activity of Pleurotus florida,” Curr. Sci., 79, 941-943,2000.
[15]	L. Stefanis, R. E. Burke, and L. A. Greene, “Apoptosis in neurodegenerative disorders,” Curr Opin Neurol, 10, 299–305, 1997.
[16]	B. Halliwell, “How to characterize an antioxidant-Anupdate,” Biochem Soc Symp, 61, 73–101, 1995.
[17]	I. B. Suffredini, H. S. Sader, A. G. Gonçalves, A. O. Reis, A. C. Gales, A. D. Varella, and R. N. Younes, “Screening of antibacterial extracts from plants native to the brazilian amazonrain forest and atlantic forest,” Braz. J. Med. Biol. Res., 37, 379–384, 2004.
[18]	B. N. Ames, M. K. Shigenaga, and T. M. Hagen, “Oxidants, antioxidants, and the degenerative diseases of aging,” Proc. Natl. Acad.Sci.,90,7915–7922,1993.
[19]	K. J. Gohil, J. A. Patel, and A. K. Gajjar. “Pharmacological Review on Centella asiatica: A Potential Herbal Cure-all,” Indian J Pharm Sci., 72(5), 546-56,2010.
[20]	D. Arora, M. Kumar, and S. D. Dubey. “Centella asiatica-A review of its medicinal uses and pharmacological effects,” Journal of Natural Remedies, 2(2), 143–149, 2002.
[21]	L. Suguna, P. Sivakumar and G. Chandrakasan, “Effect of Centella Asiatica Extract on Dermal Wound Healing in Rats,” Indian J. Exp. Biol., 34,1208-1211,1996.
[22]	H. Rosen, A. Blumenthal, and J. McCallum, “Effect of asiaticoside on wound healing in rats,” Experimental Medicine and Surgery, 125, 279–280,1967.
[23]	K. Nalini, A. R. Aroor, K. S. Karanth and A. Rao, “Effect of Centella Asiatica Fresh Leaf Aqueous Extracton Learning Memory and Biogenic Amine Turnover in Albino Rats,” Fitoterapia, 63, 232-237, 1992.
[24]	A.Yoshinori, M. Reiko, and T. Tsumematsu, “Mono and sesquiterpenoids from hydrocotyle and Centella species,” Phytochemistry, 21, 2590–2592, 1982.
[25]	S.C. CH, M. Haritha, B. S. Rao, V. Sharanand, V. Meena, “Pharmacognostic and Pharmacological Aspects of Centella Asiatica,” Int. J. Chem. Sci., 9(2), 784-794, 2011.
[26]	F. X. Maquart, G. Bellon, P. Gillery, Y. Wegrowski, and J. P. Borel, “Stimulation of collagen synthesis in fibroblast cultures by a triterpene extracted from Centella asiatica,” Connective Tissue Research, 24, 107–120, 1990.
[27]	Y. Akita, Y. Yang, T. Kawai, K. Kinoshita, K. Koyama, and K. Takahashi, “New assay method for survey in antiemetic compounds from natural sources,” Nat Prod Sci., 4, 72–77, 1998.
[28]	N. Sreejayan, and M. N. A. Rao, “Freeradical scavenging activity of curcuminoids,” Drug Res., 46, 169–171, 1997.
[29]	F. Pittella, R. C. Dutra, D. D. Junior, M. T. P. Lopes and N. R. Barbosa, “Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.,” Int J Mol Sci., 10(9), 3713–3721, 2009.
[30]	J. Harwig, and P. M. Scott, “Brineshrimp (Artemia salina L.) larvaesa screening system for fungal toxins,” Appl Microbiol, 21(6), 1011–6,1971.
[31]	B. N. Meyer, N. R. Ferrigni, J. E. Putnam, J. E. Jacobsen, D. E. Nichols, and J. L. McLaughlin, “Brineshrimp: A convenient general bioassay for active plants constituents,” J Med Plant Res., 45, 31–34, 1982.
[32]	S. Hossain, G. Kader, F. Nikkon, and T. Yeasmin, “Cytotoxicity of the rhizomes of medicinal plants,” Asian Pac J Trop Biomed, 2(2), 125–127, 2012.
[33]	T. Arumugam, M. Ayyanar, Y. J. Koil Pillai, and T. Sekar, “Phytochemical screening and antibacterial activity of leaf and callus extracts of Centella asiatica,” Bangladesh Journal of Pharmacology, 6(1), 55–60, 2011.
[34]	R. P. Samy, and I. V. T. K. Chow, “Antimicrobial and Phytochemical Analysis of Centella asiatica (L.),” Nature Precedings, 11(37), 1-6, 2011.
[35]	M. D. Fellows and M. R. O’Donovan,“Cytotoxicity in cultured mammalian cells is a function of the method used to estimate it.,” Mutagenesis,22(4), 275-280, 2007.
[36]	M. R. A. Mans, A. B. D. Rocha and G. Schwartsmann, “Anti-cancer drug discovery and development in Brazil: Targeted plant collection as rational strategy to acquire candid ateanti-cancerc ompounds,” The Oncologist, 5, 185-198, 2000.
[37]	M.N. Amin, S. Banik, M. Ibrahim, M. M. R. Moghal, M. S. Majumder, R. Siddika, M. K. Alam, K. M. R. M. Jitu, S. N. Anonna. “A Study on Ardisia solanacea for Evaluation of Phytochemical and Pharmacological Properties,” International Journal of Pharmacognosy and Phytochemical Research, 7(1), 8-15, 2015.
[38]	M. S. Majumder, M. N. Amin, M. M. R. Moghal, S. Banik, A. Kar, and M. M. Hossain. “Anthelmintic and Cytotoxic Activities of Two Medicinal Plants: Polygonum viscosum and Aphanamixis polystachya Growing in Bangladesh,” J. Sci. Res., 6(2), 339-345,2014.
[39]	T. D. Babu, G. Kuttan and J. Padikkala, “Cytotoxic and anti-tumor properties of certain taxa of Umbelliferae with special references to Centella asiatica (L.) Urban,” Journal of Ethnopharmacology, 48(1), 53-57, 1995.
[40]	M. Rahman, S. Hossain, A. Rahaman, N. Fatima, T. Nahar, B. Uddin and M.A. Basunia, “Antioxidant Activity of Centella asiatica (Linn.) Urban: Impact of Extracti on Solvent Polarity,” Journal of Pharmacognosy and Phytochemistry, 1(6), 27-32, 2013.
[41]	Rishikesh, D. R. Ghosh, M. A. Islam, S. M. S. Islam, M. M. Rahman, “Brine Shrimp Lethality Bioassay Of Methanolic Extract Of Centella asiatica Leaves,” Int. Res J Pharm. App Sci., 2(5), 39-44, 2012.
[42]	K. M. R. M. Jitu, D. Debnath, S. Asad, R. C. Das, and A. Sultana. “Phytochemical screening and evaluation of cytotoxic activity of Pandanus fascicularis L.(Fruits),” Discovery Phytomedicine, 4(3), 1-4, 2017.

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APA Style

Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. (2018). Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant, 6(1), 1-7. https://doi.org/10.11648/j.plant.20180601.11

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ACS Style

Md. Shohel Hossain; Fahmida Abdullah Tuly; Sharmin Akter; Md Saiful Islam Arman; Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018, 6(1), 1-7. doi: 10.11648/j.plant.20180601.11

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AMA Style

Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018;6(1):1-7. doi: 10.11648/j.plant.20180601.11

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@article{10.11648/j.plant.20180601.11,
  author = {Md. Shohel Hossain and Fahmida Abdullah Tuly and Sharmin Akter and Md Saiful Islam Arman and Md. Abdul Aziz and Md. Ekhtear Mahmud and Palash Das and Mohammad Hasem Babu and Md. Monirul Islam},
  title = {Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica},
  journal = {Plant},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.plant.20180601.11},
  url = {https://doi.org/10.11648/j.plant.20180601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20180601.11},
  abstract = {This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).},
 year = {2018}
}

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TY - JOUR
T1 - Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica
AU - Md. Shohel Hossain
AU - Fahmida Abdullah Tuly
AU - Sharmin Akter
AU - Md Saiful Islam Arman
AU - Md. Abdul Aziz
AU - Md. Ekhtear Mahmud
AU - Palash Das
AU - Mohammad Hasem Babu
AU - Md. Monirul Islam
Y1 - 2018/04/13
PY - 2018
N1 - https://doi.org/10.11648/j.plant.20180601.11
DO - 10.11648/j.plant.20180601.11
T2 - Plant
JF - Plant
JO - Plant
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2331-0677
UR - https://doi.org/10.11648/j.plant.20180601.11
AB - This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).
VL - 6
IS - 1
ER -

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Author Information

Md. Shohel Hossain

Department of Pharmacy, Manarat International University, Dhaka, Bangladesh
Fahmida Abdullah Tuly

Department of Pharmacy, Manarat International University, Dhaka, Bangladesh
Sharmin Akter

Department of Pharmacy, Manarat International University, Dhaka, Bangladesh
Md Saiful Islam Arman

Department of Pharmacy, Manarat International University, Dhaka, Bangladesh
Md. Abdul Aziz

Department of Pharmacy, Manarat International University, Dhaka, Bangladesh
Md. Ekhtear Mahmud

Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh
Palash Das

Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh
Mohammad Hasem Babu

Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh
Md. Monirul Islam

Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh

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APA Style

Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. (2018). Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant, 6(1), 1-7. https://doi.org/10.11648/j.plant.20180601.11

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ACS Style

Md. Shohel Hossain; Fahmida Abdullah Tuly; Sharmin Akter; Md Saiful Islam Arman; Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018, 6(1), 1-7. doi: 10.11648/j.plant.20180601.11

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AMA Style

Md. Shohel Hossain, Fahmida Abdullah Tuly, Sharmin Akter, Md Saiful Islam Arman, Md. Abdul Aziz, et al. Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica. Plant. 2018;6(1):1-7. doi: 10.11648/j.plant.20180601.11

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@article{10.11648/j.plant.20180601.11,
  author = {Md. Shohel Hossain and Fahmida Abdullah Tuly and Sharmin Akter and Md Saiful Islam Arman and Md. Abdul Aziz and Md. Ekhtear Mahmud and Palash Das and Mohammad Hasem Babu and Md. Monirul Islam},
  title = {Determination of Antiemetic, Antimicrobial, Anti-Radical and Cytotoxic Activity of Methanolic Extracts of Centella asiatica},
  journal = {Plant},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.plant.20180601.11},
  url = {https://doi.org/10.11648/j.plant.20180601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20180601.11},
  abstract = {This study was conducted to investigate the antiemetic, antimicrobial, antioxidant and cytotoxic activity of methanolic extracts of Centella asiatica. The antiemetic assay was carried out by using chick emetic model with minor modifications by calculating the mean decrease in the number of retching. The antimicrobial activity of the crude extract was performed by Disc Diffusion method. The anti-radical activity was determined by the 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) method. Brine shrimp lethality bioassay is done to determine cytotoxic activity. The anti-emetic activity of Centella asiatica leaves on young chicks revealed that these extracts have a less anti-emetic effect. The group of chicks treated with Chlorpromazine was found to have 38.4 retches as compared to the 60.4 retches of the control group, thus Chlorpromazine reduced the retches by 36.96%. The chickens treated with leaves extracts inhibited the retches up to 15.67%. The manimum antimicrobial effect was found in this methanol crude extract. The extract did not appear potent in terms of both zones of inhibition and spectrum of activity. In anti-radical activity test, the extract showed moderate free radical scavenging activity with IC50 value 241.71 μg/ml. while compared to that of the reference standard ascorbic acid. Moreover, the methanolic crude extracts also possess moderate cytotoxic principles potential (LC50 value of 39.06 μg/ml) comparing with that of standard vincristine (0.839 μg/ml).},
 year = {2018}
}

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