To investigate the mechanism of valsartan protecting podocytes and inhibiting renal injury in diabetic rats. The rat model of diabetic nephropathy was induced by combination of valsartan and high-sugar and high-fat diet. The urinary protein content, renal index, inflammatory and antioxidant indexes in the kidney, renal pathological changes and podocyte holes were investigated. Membrane WT1 and P-Cadherin protein expression levels. Compared with the model group, the 24h urine protein content of valsartan was significantly decreased (P<0.05). Valsartan significantly inhibited the body weight of the model group (P<0.01), and significantly inhibited The increase of renal index (P<0.05); the high and middle doses of valsartan could significantly reduce the levels of IL-β, TNF-α and IL-6 in rat kidney (P<0.05-0.01). The valsartan high and middle dose groups significantly reduced MDA content in rat kidney (P<0.05), and significantly increased SOD activity (P<0.05). HE and PAS staining showed that valsartan was used in model group rats. The pathological changes were alleviated, and the glomerular morphology returned to normal. The protein expression of WT1 and P-Cadherin in the kidney of DN rats by Western blot showed that P- in the kidney tissue of valsartan rats. The expression levels of Cadherin and WT1 protein were significantly increased (P<0.05). Valsartan can regulate the expression of podocyte membrane proteins WT1 and P-Cadherin to protect podocytes, and then repair renal function and anti-diabetic nephropathy.
| Published in | American Journal of Life Sciences (Volume 6, Issue 3) |
| DOI | 10.11648/j.ajls.20180603.12 |
| Page(s) | 47-51 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Valsartan, Diabetic Nephropathy, Podocyte, P-Cadherin, WT1 Protein
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APA Style
Qingfen Wang, Rui Li, Weiwei Li, Lei Wang. (2018). Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy. American Journal of Life Sciences, 6(3), 47-51. https://doi.org/10.11648/j.ajls.20180603.12
ACS Style
Qingfen Wang; Rui Li; Weiwei Li; Lei Wang. Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy. Am. J. Life Sci. 2018, 6(3), 47-51. doi: 10.11648/j.ajls.20180603.12
AMA Style
Qingfen Wang, Rui Li, Weiwei Li, Lei Wang. Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy. Am J Life Sci. 2018;6(3):47-51. doi: 10.11648/j.ajls.20180603.12
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Download@article{10.11648/j.ajls.20180603.12,
author = {Qingfen Wang and Rui Li and Weiwei Li and Lei Wang},
title = {Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy},
journal = {American Journal of Life Sciences},
volume = {6},
number = {3},
pages = {47-51},
doi = {10.11648/j.ajls.20180603.12},
url = {https://doi.org/10.11648/j.ajls.20180603.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20180603.12},
abstract = {To investigate the mechanism of valsartan protecting podocytes and inhibiting renal injury in diabetic rats. The rat model of diabetic nephropathy was induced by combination of valsartan and high-sugar and high-fat diet. The urinary protein content, renal index, inflammatory and antioxidant indexes in the kidney, renal pathological changes and podocyte holes were investigated. Membrane WT1 and P-Cadherin protein expression levels. Compared with the model group, the 24h urine protein content of valsartan was significantly decreased (P<0.05). Valsartan significantly inhibited the body weight of the model group (P<0.01), and significantly inhibited The increase of renal index (P<0.05); the high and middle doses of valsartan could significantly reduce the levels of IL-β, TNF-α and IL-6 in rat kidney (P<0.05-0.01). The valsartan high and middle dose groups significantly reduced MDA content in rat kidney (P<0.05), and significantly increased SOD activity (P<0.05). HE and PAS staining showed that valsartan was used in model group rats. The pathological changes were alleviated, and the glomerular morphology returned to normal. The protein expression of WT1 and P-Cadherin in the kidney of DN rats by Western blot showed that P- in the kidney tissue of valsartan rats. The expression levels of Cadherin and WT1 protein were significantly increased (P<0.05). Valsartan can regulate the expression of podocyte membrane proteins WT1 and P-Cadherin to protect podocytes, and then repair renal function and anti-diabetic nephropathy.},
year = {2018}
}
TY - JOUR T1 - Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy AU - Qingfen Wang AU - Rui Li AU - Weiwei Li AU - Lei Wang Y1 - 2018/12/06 PY - 2018 N1 - https://doi.org/10.11648/j.ajls.20180603.12 DO - 10.11648/j.ajls.20180603.12 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 47 EP - 51 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20180603.12 AB - To investigate the mechanism of valsartan protecting podocytes and inhibiting renal injury in diabetic rats. The rat model of diabetic nephropathy was induced by combination of valsartan and high-sugar and high-fat diet. The urinary protein content, renal index, inflammatory and antioxidant indexes in the kidney, renal pathological changes and podocyte holes were investigated. Membrane WT1 and P-Cadherin protein expression levels. Compared with the model group, the 24h urine protein content of valsartan was significantly decreased (P<0.05). Valsartan significantly inhibited the body weight of the model group (P<0.01), and significantly inhibited The increase of renal index (P<0.05); the high and middle doses of valsartan could significantly reduce the levels of IL-β, TNF-α and IL-6 in rat kidney (P<0.05-0.01). The valsartan high and middle dose groups significantly reduced MDA content in rat kidney (P<0.05), and significantly increased SOD activity (P<0.05). HE and PAS staining showed that valsartan was used in model group rats. The pathological changes were alleviated, and the glomerular morphology returned to normal. The protein expression of WT1 and P-Cadherin in the kidney of DN rats by Western blot showed that P- in the kidney tissue of valsartan rats. The expression levels of Cadherin and WT1 protein were significantly increased (P<0.05). Valsartan can regulate the expression of podocyte membrane proteins WT1 and P-Cadherin to protect podocytes, and then repair renal function and anti-diabetic nephropathy. VL - 6 IS - 3 ER -
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