| Peer-Reviewed

Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia

Received: 11 June 2015     Accepted: 26 June 2015     Published: 23 March 2016
Views:       Downloads:
Abstract

Hydrogen cyanide is a highly toxic volatile compound which interferes with the cellular respiration by inhibiting the cytochrome oxidase enzyme in mitochondria. Root area of crops were consisting several bacteria that may have beneficial aspects. The objective of this study was to investigate the qualitative capability of hydrogen cyanide production by Pseudomonas fluorescens species isolates. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. Twelve Pseudomonas fluorescens species were isolated from the Rhizosphere of Fabaceae family namely faba bean screened for the production of hydrogen cyanide. For the production of hydrogen cyanide, Pseudomonas fluorescens isolates were streaked into King’s B agar plates supplemented with glycine. Total of Pseudomonas fluorescens isolates had a potential to produce hydrogen cyanide. Therefore, these isolated can be used as a biological control against plant pathogen microbes.

Published in American Journal of Life Sciences (Volume 4, Issue 2)
DOI 10.11648/j.ajls.20160402.11
Page(s) 13-19
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Biological Control, Faba Bean, Hydrogen Cyanide, Pseudomonas fluorescens

References
[1] Antoun, H., Beauchamp, C. J., Goussard, N., Chabot, R. and Lalande, R. (1998). Potential of Rhizobium and Bradyrhizobium species as a plant growth promoting rhizobacteria on non legumes. Plant Soil 204: 57-67.
[2] Askeland, R. A. and Morrison, S. M. (1983). Cyanide production by Pseudomonas fluorescens and Pseudomonas aeruginosa. Appl. Environ. Microbiol. 45: 1802-1807.
[3] Bakker, A. W. and Schippers, B. (1987). Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol. Biochem.19: 451-457.
[4] Bano, N. and Musarrat, J. (2003). Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Curr Microbiol 46: 324-328.
[5] Castric, P. (1977). Glycine metabolism of Pseudomonas aeruginosa: Hydrogen cyanide biosynthesis. J. Bacteriol.130: 826-831.
[6] Castric, P. A. (1975). Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Can. J. Microbiol. 21: 613-618.
[7] Cheeke, P. R. (1995). Endogenous toxins and mycotoxins in forage grasses and their effects on livestock. J Ani Sci 73: 909-918.
[8] Corbett, J. R., (1974). Pesticide design. In: The Biochemical Mode of Action of Pesticides, Academic Press, Inc., London, pp.44-86.
[9] Defago, G., Berling, C. H., Borger, U., Keel, C. and Voisard, C. (1990). Suppression of black rot of tobacco by a Pseudomonas strain: Potential applications and mechanisms. In: Biological Control of Soil Borne Plant Pathogen, pp. 93-108, (Hornby, D., Cook, R. J. and Henis, Y., eds). CAB International, Wellingford.
[10] Defago, G., Berling, C. H., Burger, U., Haas, D., Kahr, G., Keel, C., Voisard, C., Wirthner, P. and Wuthrich, B. (1990). Suppression of black root rotof tobacco and other root diseases by strains of Pseudomonas fluorescnes: Potential applications and mechanisms. In: Biological Control of Soil Borne Plant Pathogens (Ed. D. Hornby) CAB International, Wallingford, Oxon, UK, pp.93-108.
[11] Duffy, B., Schouten, A. and Raajimakers, J. (2003). Pathogen selfdefense: mechanisms to counteract microbial antagonism. Annu. Rev. Phytopathol., 45: 501-538.
[12] Dwived, D. and Johri, B.N. (2003). Antifungals from fluorescent pseudomonads: biosynthesis and regulation. Curr Sci. 85:1693-1703.
[13] Ebbs, S. (2004). Biological degradation of cyanide compounds. Curr Opin Biotechnol 15: 231-236.
[14] Flaishman, M. A., Eyal, Z., Zilberstein, A., Voisard, C. and Haas, D. (1996). Suppression of Septoria triticiblotch and leaf rust of wheat by recombinant cyanide producing strains of Pseudo-monas putida. Mol. Plant-Microbe Interact. 9: 642-645.
[15] Gehring, P. J., Mohan, R. J. and Watamare, P. G., (1993). Solvents, fumigants and related compounds. In: Handbook of Pesticide Toxiocology, Vol. 2, Eds., Hayes, W.J. and Laws, E.R., Academic Press, inc., San Diego, California, pp. 646-649.
[16] Guo, Y., Zheng, H., Yang, Y. and Wang, H. (2007). Characterization of Pseudomonas corrugata strain p94 isolated from soil in Beijing as a potential bicontrol agent. Curr. Microbiol. 55: 247-253.
[17] Keel, C., Voisard, C., Berling, C. H., Kahr, G. and Defago, G. (1989). Iron sufficiency, a pre-requisite for the suppression of tobacco black root rot by Pseudomonas fluoresens strain CHAO under gnobiotic conditions. Phytopathol. 79: 584-589.
[18] King, E. O., Ward, M. K. and Raney, D. E. (1954). Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med., 44: 301-307.
[19] Lorck, H. (1948). Production of hydrocyanic acid by bacteria. Physiol. Plant 1: 142-146.
[20] Luque-Almagro, V. M., Huertas, M. J., Martinez-Luque, M., Moreno-Vivian, C., Roldan, M. D., Garcia-Gil, L. J., Castillo, F. and Blasco, R. (2005). Bacterial degradation of cyanide and its metal complexes under alkaline conditions. Appl Environ Microbiol 71(2): 940-947.
[21] McMahon, J. M., White, W. L. B. and Sayre, R. T. (1995). Cyanogenesis in cassava (Manihot esculantaCrantz). J Exp Bot 46: 731-741.
[22] Muleta, D., Assefa, F.and Granhall. U. (2007). In vitro Antagonism of rhizobacteria isolated from Coffea arabica L. against emerging fungal coffee pathogens. Eng. Life Sci.7: 577-586.
[23] Nagarajkumar, M., Bhaskaran, R. and Velazhahan, R. (2004). Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen. Microbiol. Res. 159: 73-81.
[24] O'Sullivan, D. J. and O'Gara, F. (1992). Traits of fluorescent Pseudomonas spp. involved in suppression of plant root patho-gens. Microbiol. Rev. 56: 662-676.
[25] Raaijmakers, J. M., Vlami, M. and de Souza, J. T. (2002). Antibiotic production by bacterial biocontrol agents. Int. J. Gen. Mol. Microbiol. 81: 537–547.
[26] Ramatte, A., Frapolli, M., Defago, G. and Moenne-Loccoz, Y. (2003). Phylogeny of HCN synthase encoding hcnbcgenes in biocontrol fluorescent pseudomonads and its relationship with host plant species and HCN synthesis ability. Molecular Biol.. Pl. Microbe Interaction 16: 525-535.
[27] Rangajaran, S., Saleena, L. M., Vasudevan, P. and Nair, S. (2003). Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Plant Soil 251: 73–82.
[28] Sacherer, P., Défago, G. and Haas, D. (1994). Extracellular protease and phosholipase C are controlled by the global regulatory gene gacA in the biocontrol strain Pseudomonas fluorescens CHA0. F. E. M. S. Microbiol. Lett. 116: 155–160.
[29] Shivakumar, B. (2007). Biocontrol Potential and Plant Growth Promotional Activity of Fluorescent Pseudomonads of Western Ghats, M.Sc. Thesis. Dharwad University of Agricultural Sciences, Dharwad.
[30] Siddiqui, I. A., Shaukat, S. S., Khan, G. H. and Ali, N. I. (2003). Supperssion of Meloidogyne javanica by Pseudomonas aeruginosa IE-6S+ in tomato: the influence of NaCl, oxygen and iron level. Soil Biol. Biochem. 35: 1625-1634.
[31] Siddiqui, I.A., Shaukat, S. S., Sheikh, I. H. and Khan, A. (2006). Role of cyanide production by Pseudomonas fluorescens CHA0 in the suppression of root-knot nematode, javanica in tomato. World J. Microbiol. Biotechnol. 22: 641-650.
[32] Stutz, E.W., Defago, G. and Kern, H. (1986). Naturally occurring fluorescent pseudomonads involved in suppression of black root rot of tobacco. Phytopathol. 76: 181-185.
[33] Voisard, C., Bull, C. T., Keel, C., Laville, J., Maurhofer, M., Schnider, U., De’fago, G. and Haas, D. (1994). Biocontrol of root diseases by Pseudomonas fluorescens CHA0: current concepts and experimental approaches. In: Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMO’s, pp. 67–89, (O’Gara, F., Dowling, D. N. and Boesten, B., eds). VCH, Weinheim.
[34] Voisard, C., Keel, C., Haas, D. and Defago, G. (1989). Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under gnotobiotic conditions. EMBO J., 8: 351-358.
[35] Wangi, F. M., Hauschild, R., Sokora, R.A. and Mutitu, E. (2002). Challenges to Organic Farming and Sustainable Land Use in the Tropics and Subtropics. Deutschea Tropentag, Witzenhausen.
[36] Wei, G., Kloepper, J. W. and Tuzun, S., 1991, Inductionof systemic resistance of cucumber to Colletotrichum orbiculareby selected strains of plant growth promoting rhizobacteria. Phytopathol., 81: 1508-1512.
[37] Weller, D. M. (1985). Application of fluorescens Pseudomonas to control root diseased. In: Ecology and Management of Soil Borne Plant Pathogens, pp.137-140, (Purker, C. S., Roura, A. D., Moore, K. J., Wang, P. T. W. and Kellmorgan J. F., eds). American Phytopathological Society, St. Paul.
[38] Weststeijn, W. A. (1990). Fluorescent pseudomonads isolate E11-2 as biological agent for Pythium root rot in tulips. Netherlands J. Pl. Pathol., 96: 262-272.
[39] Zagrobelny, M., Bak, S. and Moller, B. L. (2008). Cyanogenesis in plants and arthropods. J Phytochem 69: 1457-1468.
Cite This Article
  • APA Style

    Fekadu Alemu. (2016). Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia. American Journal of Life Sciences, 4(2), 13-19. https://doi.org/10.11648/j.ajls.20160402.11

    Copy | Download

    ACS Style

    Fekadu Alemu. Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia. Am. J. Life Sci. 2016, 4(2), 13-19. doi: 10.11648/j.ajls.20160402.11

    Copy | Download

    AMA Style

    Fekadu Alemu. Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia. Am J Life Sci. 2016;4(2):13-19. doi: 10.11648/j.ajls.20160402.11

    Copy | Download

  • @article{10.11648/j.ajls.20160402.11,
      author = {Fekadu Alemu},
      title = {Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {2},
      pages = {13-19},
      doi = {10.11648/j.ajls.20160402.11},
      url = {https://doi.org/10.11648/j.ajls.20160402.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160402.11},
      abstract = {Hydrogen cyanide is a highly toxic volatile compound which interferes with the cellular respiration by inhibiting the cytochrome oxidase enzyme in mitochondria. Root area of crops were consisting several bacteria that may have beneficial aspects. The objective of this study was to investigate the qualitative capability of hydrogen cyanide production by Pseudomonas fluorescens species isolates. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. Twelve Pseudomonas fluorescens species were isolated from the Rhizosphere of Fabaceae family namely faba bean screened for the production of hydrogen cyanide. For the production of hydrogen cyanide, Pseudomonas fluorescens isolates were streaked into King’s B agar plates supplemented with glycine. Total of Pseudomonas fluorescens isolates had a potential to produce hydrogen cyanide. Therefore, these isolated can be used as a biological control against plant pathogen microbes.},
     year = {2016}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Isolation of Pseudomonas flurescens from Rhizosphere of Faba Bean and Screen Their Hydrogen Cyanide Production Under in Vitro Stduy, Ethiopia
    AU  - Fekadu Alemu
    Y1  - 2016/03/23
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajls.20160402.11
    DO  - 10.11648/j.ajls.20160402.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 13
    EP  - 19
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20160402.11
    AB  - Hydrogen cyanide is a highly toxic volatile compound which interferes with the cellular respiration by inhibiting the cytochrome oxidase enzyme in mitochondria. Root area of crops were consisting several bacteria that may have beneficial aspects. The objective of this study was to investigate the qualitative capability of hydrogen cyanide production by Pseudomonas fluorescens species isolates. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. Twelve Pseudomonas fluorescens species were isolated from the Rhizosphere of Fabaceae family namely faba bean screened for the production of hydrogen cyanide. For the production of hydrogen cyanide, Pseudomonas fluorescens isolates were streaked into King’s B agar plates supplemented with glycine. Total of Pseudomonas fluorescens isolates had a potential to produce hydrogen cyanide. Therefore, these isolated can be used as a biological control against plant pathogen microbes.
    VL  - 4
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Department of Biology, College of Natural and Computational Sciences, Dilla University, Dilla, Ethiopia

  • Sections