Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Owa Olugbenga Stephen; Ucheabba Destinee Esther

doi:doi:10.11648/j.ajbio.20150305.14

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Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther

Published in American Journal of BioScience (Volume 3, Issue 5)

Received: 19 June 2015 Accepted: 29 June 2015 Published: 19 August 2015

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Abstract

Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose

Published in	American Journal of BioScience (Volume 3, Issue 5)
DOI	10.11648/j.ajbio.20150305.14
Page(s)	183-189
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Testes, Brown Eggshell, Potassium Bromate, Histopathology

References

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Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther. (2015). Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. American Journal of BioScience, 3(5), 183-189. https://doi.org/10.11648/j.ajbio.20150305.14

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ACS Style

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Owa Olugbenga Stephen; Ucheabba Destinee Esther. Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. Am. J. BioScience 2015, 3(5), 183-189. doi: 10.11648/j.ajbio.20150305.14

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AMA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther. Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. Am J BioScience. 2015;3(5):183-189. doi: 10.11648/j.ajbio.20150305.14

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@article{10.11648/j.ajbio.20150305.14,
  author = {Charles Obiora Nwonuma and Emenike Onyebum Irokanulo and Owa Olugbenga Stephen and Ucheabba Destinee Esther},
  title = {Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {5},
  pages = {183-189},
  doi = {10.11648/j.ajbio.20150305.14},
  url = {https://doi.org/10.11648/j.ajbio.20150305.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150305.14},
  abstract = {Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose},
 year = {2015}
}

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TY  - JOUR
T1  - Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats
AU  - Charles Obiora Nwonuma
AU  - Emenike Onyebum Irokanulo
AU  - Owa Olugbenga Stephen
AU  - Ucheabba Destinee Esther
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150305.14
DO  - 10.11648/j.ajbio.20150305.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 183
EP  - 189
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150305.14
AB  - Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose
VL  - 3
IS  - 5
ER  -

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Author Information

Charles Obiora Nwonuma

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Emenike Onyebum Irokanulo

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Owa Olugbenga Stephen

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Ucheabba Destinee Esther

Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria

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Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther. (2015). Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. American Journal of BioScience, 3(5), 183-189. https://doi.org/10.11648/j.ajbio.20150305.14

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ACS Style

Charles Obiora Nwonuma; Emenike Onyebum Irokanulo; Owa Olugbenga Stephen; Ucheabba Destinee Esther. Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. Am. J. BioScience 2015, 3(5), 183-189. doi: 10.11648/j.ajbio.20150305.14

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AMA Style

Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther. Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats. Am J BioScience. 2015;3(5):183-189. doi: 10.11648/j.ajbio.20150305.14

Copy | Download

@article{10.11648/j.ajbio.20150305.14,
  author = {Charles Obiora Nwonuma and Emenike Onyebum Irokanulo and Owa Olugbenga Stephen and Ucheabba Destinee Esther},
  title = {Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats},
  journal = {American Journal of BioScience},
  volume = {3},
  number = {5},
  pages = {183-189},
  doi = {10.11648/j.ajbio.20150305.14},
  url = {https://doi.org/10.11648/j.ajbio.20150305.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150305.14},
  abstract = {Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose},
 year = {2015}
}

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TY  - JOUR
T1  - Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats
AU  - Charles Obiora Nwonuma
AU  - Emenike Onyebum Irokanulo
AU  - Owa Olugbenga Stephen
AU  - Ucheabba Destinee Esther
Y1  - 2015/08/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbio.20150305.14
DO  - 10.11648/j.ajbio.20150305.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 183
EP  - 189
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20150305.14
AB  - Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose
VL  - 3
IS  - 5
ER  -

Copy | Download